KEY WORDS | Biochemistry, Cell Biology, Neuroscience |
CITY | Barcelona |
COUNTRY | Spain |
DETAILS OF THE OFFER | The PhD candidate will be involved in deciphering the contribution of distinct progenitor pools to specific functional neuronal circuits during hindbrain morphogenesis. For this, he/she will combine intersectional genetic fate mapping, in vivo imaging and the use of the 3D-atlas of neuronal differentiation previously built up in the lab [Blanc et al, eLife 2022]. The progenitor-restricted intersectional fate mapping approach facilitates the exclusive genetic labelling of de?ned neural progenitors and their progeny based on the expression of two genes. In our case, we will select different progenitor cell populations by the expression of a given proneural gene and the differentiated neurons will be identified by expression of the pan-differentiation marker HuC, or by specific glutamatergic or GABAergic neurotransmitters makers. Since there is contradictory data about the progenitor origin of the glutamatergic and GABAergic neuronal populations, these experiments will allow to determine whether
He/she will dissect the gene regulatory network operating in the generation of specific mature neuronal populations by investigating whether we can predict changes in cell progenitor states by the specific transcriptional signature over time. We will use two approaches: RNA-seq of specific progenitor populations, and Hybridization Chain Reaction profiling that allows multiplexed quantitative analysis of mRNA expression with high resolution within intact specimens. |
TYPE OF JOB | PhD |
INFORMATION CONTACT |